Next generation functional genomics

Polyploid species present difficulties for mutagenesis, requiring multiple mutations in each homeologous gene. The flexibility of hairpinRNAi can be exploited to target all homeologous genes with a single hairpin construct.

The study of gene interactions may require pyramiding additional mutations on top of the first mutation used to identify the gene of interest. CSIRO’s hairpinRNAi technology is ideal for studying gene interactions. Its speed and efficiency make it the technology of choice to create targeted second mutations in candidate genes.

HairpinRNAi technology is proving to be a key technology for the study of complex secondary metabolism in non model plants. Genes encoding enzymes are identified and their functions confirmed. RNAi knock out mutations can also identify flux control points in the pathway.