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Naturally occuring antisense transcripts in stress responsive gene control

Supervisor: Dr Narayana Upadhyaya (02 6246 5491)
Location: Canberra
Status: open

Abiotic stress adaptation in crops is currently one of the most-active topics in basic and applied biology, particularly because of impending global adverse climate changes. Several different sets of cis- and trans-acting factors are known to be involved in stress-responsive gene control allowing the plants to thrive and produce under stress. The association of natural antisense transcripts (NATs) and production and activity of small interfering RNA (siRNA) with such gene control has recently been demonstrated.

A CustomArray will be used to study the differential expression of NAT gene pairs during drought stress to identify induced NATs. Functional validation of NAT-derived siRNA will be carried out with knockout and knockdown mutants. Identified stress responsive genes could be used in classical and/or transformation breeding for stress tolerance.

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