Publications

Waterhouse, P.M., Graham, M.W. and Wang, M.-B. (1998) Virus resistance and gene silencing in plants is induced by double-stranded RNA. Proc. Nat. Acad. Sci USA. 95 13959-13964.

Waterhouse, P.M., Smith, N.A. and Wang, M.-B. (1999) Virus resistance and gene silencing: killing the messenger. Trends in Plant Science 4: 452-457.

Wang, M.-B. and Waterhouse, P.M. (2000) High-efficiency silencing of a b-glucuronidase gene in rice is correlated with repetitive transgene structure but independent of DNA methylation. Plant Molecular Biology  43, 67-82.

Wang, M.-B., Abbott, D. and Waterhouse, P.M. (2000) A single copy of a virus derived transgene encoding hairpin RNA gives immunity to barley yellow dwarf virus. Molecular Plant Pathology 1, 401-10.

Smith, N.A., Singh, S.P., Wang, M.-B., Stoutjesdijk, P., Green, A. and Waterhouse, P.M. (2000) Total silencing by intron-spliced hairpin RNAs. Nature 407, 319-320.

Waterhouse, P.M., Wang, M.-B. and Lough T. (2001) Gene silencing as an adaptive defence against viruses.  Nature 411 834-842.

Wesley, S.V., Helliwell, C., Smith, N.A., Wang, M.-B., Rouse, D., Liu, Q., Gooding, P., Singh, S., Abbott, D., Stoutjesdijk, P., Robinson, S., Gleave A., Green, A., and Waterhouse, P.M. (2001) Constructs for Efficient, Effective and High Throughput Gene Silencing in Plants. The Plant Journal 27, 581-590.

Wang, M.-B., Wesley, V., Finnegan, J., Smith, N. and Waterhouse, P.M. (2001) Replicating satellite RNA induces sequence-specific DNA methylation and truncated transcripts in plants. RNA 7, 16-28.

Waterhouse, P.M, Wang M.-B. and Finnegan, E.J. (2001) The role of short RNAs in gene silencing. Trends in Plant Science 6, 297-302.

Finnegan, E.J., Wang, M,-B. and Waterhouse, P.M. (2001) Gene Silencing: Fleshing out the bones. Current Biology 11, 99-102.

Helliwell, C., Wesley, S.V., Wielopolska, .A.J. and Waterhouse, P.M. (2002) High throughput vectors for efficient gene silencing in plants.  Functional Plant Biology 29, 1217-122.

Stoutjesdijk, P.A., Singh, S.P., Liu, Q., Hurlstone, C.J., Waterhouse, P.M. and Green, A.G. (2002) hpRNA-mediated targeting of the Arabidopsis FAD2 gene gives highly efficient and stable silencing. Plant Physiology. 129, 1723-31. 

Beclin, C., Boutet, S., Waterhouse, P. and Vaucheret, H. (2002)  A branched pathway for transgene-induced RNA silencing in plants. Current Biology 12, 684­688.

Horser, C., Abbott, D., Wesley, S.V., Smith, N., and Waterhouse, P.M. (2002) Gene silencing – principles and application. Genetic Engineering 24, 239-256.

Wang, M.-B. and Waterhouse, P.M. (2002) Application of gene silencing in plants. Current Opinion in Plant Biology  5, 146-150.

Liu, Q., Singh, S.P. and Green, A.G. (2002)  High-stearic and high-oleic cottonseed oils produced by hairpin RNA-mediated post-transcriptional gene silencing.  Plant Physiology 129, 1732-1743.

Finnegan, E.J.,  Margis, R. and Waterhouse, P.M. (2003) Posttranscriptional Gene Silencing Is Not Compromised in the Arabidopsis CARPEL FACTORY (DICER-LIKE1) Mutant, a Homolog of Dicer-1 from Drosophila. Current Biology 13, 236-40. 

Wesley, S.V., Liu Q., Wielopolska A., Ellacott G., Smith N., Singh, S, and Helliwell C. (2003) Custom knockouts with hairpinRNA mediated silencing. Chapter in Plant Functional Genomics (Erich Grotewold, ed.) Humana Press New Jersey USA.

Helliwell, C. and Waterhouse, P. (2003) Constructs and methods for high-throughput gene silencing in plants.  Methods 30, 289-95.

 Waterhouse, P.M. and Helliwell, C.A. (2003) Exploring plant genomes by RNA-induced gene silencing.  Nature Reviews: Genetics 4, 29-38.

Allen, R.S., Millgate, A.G., Chitty, J.A., Thistleton, J., Miller, J.A.C., Fist, A.J., Gerlach, W.L. and Larkin, P.J. (2004) RNAi-mediated replacement of morphine with the nonnarcotic alkaloid reticuline in opium poppy.  Nature Biotechnology 22, 1559-1566.

Aili Lu, Huanqing Zhang, Xiaoyan Zhang, Hongxia Wang, Qikuan Hu, Li Shen, Brian S. Schaffhausen, Weimin Hou and Linsong Li. (2004) Attenuation of SARS coronavirus by a short hairpin RNA expression plasmid targeting RNA-dependent RNA polymerase. Virology 324, 84-89.

Hironori Nishitsuji, Tamako Ikeda, Hiroyuki Miyoshi, Takashi Ohashi, Mari Kannagi and Takao Masuda. (2004) Expression of small hairpin RNA by lentivirus-based vector confers efficient and stable gene-suppression of HIV-1 on human cells including primary non-dividing cells. Microbes and Infection 6, 76-85.

Spankuch, B., Matthess, Y., Knecht, R., Zimmer, B., Kaufmann, M., Strebhardt., K. (2004) Cancer inhibition in nude mice after systemic application of U6 promoter-driven short hairpin RNAs against PLK1. J Natl Cancer Inst. Jun 2; 96(11): 862-72.

Andrea Ventura, Alexander Meissner, Christopher P. Dillon, Michael McManus, Phillip A. Sharp, Luk Van Parijs, Rudolf Jaenisch, and Tyler Jacks. (2004) Cre-lox-regulated conditional RNA interference from transgenes. Proc Natl Acad Sci U S A. July 13, 101(28): 10380–10385.

Wesley, S.V., Helliwell, C., Wang M.-B. and Waterhouse, P. (2004) Posttranscriptional Gene Silencing in Plants. Chapter 7 in RNA Interference, Editing, and Modification (Jonatha M. Gott, ed.) Humana Press New Jersey USA.

Helliwell C. and Waterhouse, P. (2005) Constructs and Methods for hairpin RNA-mediated Gene Silencing in Plants. Methods in Enzymology 392, RNA Interference, edited by David R. Engelke and John Rossi (scheduled publication date of February 1, 2005).

Huang, B., and Kochanek, S. (2005) Adenovirus-mediated silencing of huntingtin expression by shRNA. Hum Gene Ther. May, 16(5): 618-26.

Millar, A.A. and Waterhouse, P.M. (2005) Plant and Animal microRNAs: similarities and differences. Functional and Integrative Genomics (scheduled publication date April 2005).

Marvin J. Grubman and Teresa de los Santos. (2005) Rapid control of foot-and-mouth disease outbreaks: is RNAi a possible solution? Trends in Immunology 26, 65-68.

Wielopolska, A., Townley, H., Moore, I., Waterhouse, P. and Helliwell, C. (2005) A high-throughput inducible RNAi vector for plants.  Plant Biotechnology Journal 3, 583-590.